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The January, 2018, issue of Cold Spring Harbor Protocols features three protocols on Gateway recombinatorial cloning for use in high-throughput studies. The Gateway recombinatorial cloning system was developed for cloning multiple DNA fragments in parallel (e.g., in 96-well formats) in

Interactions between RNA binding proteins (RBPs) and mRNAs are critical to post-transcriptional gene regulation. Eukaryotic genomes encode thousands of mRNAs and hundreds of RBPs. However, in contrast to interactions between transcription factors (TFs) and DNA, the interactome between RBPs and

The December, 2016, issue of Cold Spring Harbor Protocols features four protocols on how to prepare reagents for yeast one-hybrid analysis. An important question when studying gene regulation is which transcription factors (TFs) interact with which cis-regulatory elements, such as