Representative publications

Koh, E. I.; Oluoch, P. O.; Ruecker, N.; Proulx, M. K.; Soni, V.; Murphy, K. C.; Papavinasasundaram, K.; Reames, C. J.; Trujillo, C.; Zaveri, A.; Zimmerman, M. D.; Aslebagh, R.; Baker, R. E.; Shaffer, S. A.; Guinn, K. M.; Fitzgerald, M.; Dartois, V.; Ehrt, S.; Hung, D. T.; Ioerger, T. R.; Rubin, E. J.; Rhee, K. Y.; Schnappinger, D.; Sassetti,C. M., Chemical-genetic interaction mapping links carbon metabolism and cell wall structure to tuberculosis drug efficacy. Proc Natl Acad Sci U S A 2022, 119 (15), e2201632119.

Bandekar AC, Subedi S, Ioerger TR, Sassetti CM. Cell-Cycle-Associated Expression Patterns Predict Gene Function in Mycobacteria. Curr Biol. 2020;30(20):3961-71 e6. Epub 2020/09/12. doi: 10.1016/j.cub.2020.07.070. PubMed PMID: 32916109; PMCID: PMC7578119

Smith CM, Baker RE, Proulx MK, Mishra BB, Long JE, Park SW, et al. Host-pathogen genetic interactions underlie tuberculosis susceptibility in genetically diverse mice. Elife. 2022;11. Epub 2022/02/04. doi: 10.7554/eLife.74419. PubMed PMID: 35112666; PubMed Central PMCID: PMC8846590. 

Kiritsy MC, McCann K, Mott D, Holland SM, Behar SM, Sassetti CM, et al. Mitochondrial respiration contributes to the interferon gamma response in antigen-presenting cells. Elife. 2021;10. Epub 2021/11/03. doi: 10.7554/eLife.65109. PubMed PMID: 34726598; PubMed Central PMCID: PMC8598164

Common Variants in the Glycerol Kinase Gene Reduce Tuberculosis Drug Efficacy. (2019). Common Variants in the Glycerol Kinase Gene Reduce Tuberculosis Drug Efficacy., 10(4). http://doi.org/10.1128/mBio.00663-19 mBio link

Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM. 2018. ORBIT: a new paradigm for genetic engineering of mycobacterial chromosomes. mBio 9:e01467-18. https://doi.org/10.1128/ mBio.01467-18. mBio link

Rittershaus, ESC Baek, SH, Krieger, IV, Nelson, SJ,Cheng, YS, Nambi, S, Baker, RE, Leszyk, JD, Shaffer, SA, Sacchettini, JS, Sassetti, CM.  A lysine acetyltransferase contributes to the metabolic adaptation to hypoxia inMycobacterium tuberculosis.  Cell Chem Bio.  Cell chem bio. 25, 1–11, 2018  Cell link

Mishra, B.B., Lovewell, R, Olive, A.J., Zhang, G., Wang, W., Eugenin, E., Smith, C.M., Yao, J.P., Long, J.E., Dubuke, M.L., Palace, S., Goguen, J.D., Baker, R.E., Nambi, S., Mishra, R., Booty, M.G., Baer, C., Shaffer, S.A., Dartois, V, McCormick, B., Chen, X., and Sassetti, C.M.  Nitric oxide prevents a pathogen permissive granulocytic inflammation during tuberculosis.  Nat Microbiol. 2017 May 15;2:17072  Pubmed

Smith CM, Proulx MK, Olive AJ, Laddy D, Mishra BB, Moss C, Gutierrez NM, Bellerose MM, Barreira-Silva P, Phuah JY, Baker RE, Behar SM, Kornfeld H, Evans TG, Beamer G, SassettiCM. Tuberculosis Susceptibility and Vaccine Protection Are Independently Controlled by Host Genotype.MBio. 2016 Sep 20;7(5). pii: e01516-16. PMID: 27651361 mBio link

Nambi, S., Long, J.E., Mishra, B.B., Baker, R., Murphy, K., Olive, A.J., Nguyen, H., Shaffer, S. A., and Sassetti, C.M.  The oxidative stress network of M. tuberculosis reveals a novel oxidoreductase complex critical for virulence.  Cell Host and Microbe.  Volume 17, Issue 6, p829–837. (2015) PMID: 26067605  Pubmed

Zhang, G., Zhou, B., Li, S., Yue, Y., Yang, H., Wen, Y., Zhan, S., Wang, W., Liao, M., Zhang, M.,Zeng, G., Feng, C. G., Sassetti, C.M., and Chen, X.  Allele-specific induction of IL-1β expression by C/EBPβ and PU.1 contributes to increased tuberculosis susceptibility.  PLoS Pathogens.  PLoS Pathog10(10): e1004426. doi:10.1371/journal.ppat.1004426.  (2014). PMID: 25329476

Padilla-Benavides T, Long JE, Raimunda D, Sassetti CM, Argüello JM. A Novel P1B-type Mn2+-transporting ATPase Is Required for Secreted Protein Metallation in Mycobacteria. J Biol Chem. 2013 Apr 19; 288(16):11334-47. View in: PubMed

Dejesus MA, Zhang YJ, Sassetti CM, Rubin EJ, Sacchettini JC, Ioerger TR. Bayesian analysis of gene essentiality based on sequencing of transposon insertion libraries. Bioinformatics. 2013 Mar 15; 29(6):695-703. View in: PubMed

Mishra BB, Rathinam VA, Martens GW, Martinot AJ, Kornfeld H, Fitzgerald KA, Sassetti CM. Nitric oxide controls the immunopathology of tuberculosis by inhibiting NLRP3 inflammasome-dependent processing of IL-1ß. Nat Immunol. 2013 Jan; 14(1):52-60. View in: PubMed

Zhang, Y.J., Ioerger, T.R., Huttenhower, C., Long, J.E., Sassetti, C.M., Sacchettini, J.C. and Rubin, E.J. Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis. Plos Pathog. PLoS Pathog 8(9): e1002946. doi:10.1371/journal.ppat.1002946.

Griffin, J.E.,, Pandey, A.K., Gilmore, S.A., Mizrahi, V., Mckinney, J.D., Bertozzi, C.B., and Sassetti, C.M. Cholesterol catabolism by Mycobacterium tuberculosis requires transcriptional and metabolic adaptations. In Press. Chemistry and Biology (2012).

Gee, C.L., Papavinasasundaram, K.G., Blair, S..R., Baer, C., Falick, A.M., King, D.S., Griffin. J., Venghatakrishnan, H., Christopher M. Sassetti* and Tom Alber*. A pseudokinase complex controls cell wall synthesis in mycobacteria. *Corresponding authors. In Press. Science Signalling. (2012).

Griffin, J.E., Gawronski, J.D., DeJesus, M.A., Ioerger, T. R., Akerley, B. A., Sassetti, C.M. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. PLoS Pathogens. 7(9): e1002251 (2011)

Baek, S., Li, A.H., Sassetti, C.M. Metabolic regulation mycobacterial growth and antibiotic sensitivity. PLoS Biol 9(5): e1001065. doi:10.1371/journal.pbio.1001065 (2011).

Coulombe, F., Divangahi, M., Veyrier, F., de Léséleuc, L., Gleason, J.L., Yang, Y., Kelliher, M.A., Pandey, A.K., Sassetti, C.M., Reed, M., Behr, M.A.. Increased NOD2-Mediated Recognition of N-Glycolyl Muramyl Dipeptide. J. Exp. Med. 206:1709-1716. (2009)

Pandey, A., Yang, Y., Jiang, Z., Fortune, S., Coulombe, F., Behr, M.A., Fitzgerald, K.A., Sassetti, C.M.*, Kelliher, M.A*. NOD2, RIP2 and IRF5 Play a Critical Role in the Type I Interferon Response to Mycobacterium tuberculosis. PLoS Pathogens. 5(7): e1000500. doi:10.1371/journal.ppat.1000500 (2009). *corresponding authors.

Pandey, A.K. and Sassetti, C.M. Mycobacterial persistence requires the utilization of host cholesterol. Proc. Nat. Acad. Sci. 105 (11):4376-4380. (2008)

Yang, Y., Yin, C., Pandey, A.K., Abbott,D., Sassetti, C.M., Kelliher, M.A. NOD2 pathway activation by MDP or Mycobacterium tuberculosis infection involves the stable polyubiquitination of Rip2. J. Biol. Chem. 282:36223-9. (2007)

Joshi, S. M., Pandey, A.K., Capite, N., Fortune, S.M., Rubin, E.J., and Sassetti, C.M. Characterization of mycobacterial virulence genes through genetic interaction mapping. Proc. Nat. Acad. Sci. 103:11760-11765. (2006)