General considerations for setting up Centrifugation Experiments

It is important that the buffer you are using does not absorb at the wavelength being used and the absorbance should be checked against water at the desired wavelength.

Samples should be tested for aggregation prior to being run in the AUC. This can be done by spinning samples in a microfuge at high speed for 20 min and making sure there is no pellet (absorbance can be measured before and after spin).

Our lab uses Ultrascan III to analyze our data. There are several options for data analysis software. Please see the suggested websites below for more options.

Ultrascan Homepage

Center for Analytical Ultracentrifugation of Macromolecular Assemblies

Beckman XLI Centrifuge


University of Connecticut Analytical Ultracentrifugation Facility
http://www.biotech.uconn.edu/auf/

RASMB
http://www.bbri.org/RASMB/rasmb.html

SedAnal
http://www.bbri.org/faculty/stafford/aucrl.html

SedFit
http://www.analyticalultracentrifugation.com/default.htm