Protocols

Antibody DNA-Hybridization

Antibody RNA-Hybridization

Culture and Fixation of Cells

DNA Hybridization

Nick translation

RNA DNA Hybridization

RNA Hybridization

Additional Resources

More in depth discussion of the development and applications of DNA/RNA in
situ hybridization methodology can be found in the following articles (PDFs):

Byron M, Hall LL, Lawrence JB. A multifaceted FISH approach to study endogenous RNAs and DNAs in native nuclear and cell structures. Curr Protoc Hum Genet. 2013 Jan;Chapter 4:Unit 4.15.

Tam, R., Shopland, L., Johnson, C.V., McNeil, J. and Lawrence, J.B, 2002. “Applications of RNA FISH for visualizing gene expression and nuclear architecture”, in the book series, FISH: A Practical Approach, Oxford University Press.

Johnson, C. V., and Lawrence, J. B. (1991). Genetic Analysis Techniques and Applications, 8, 75.

McNeil, J. A., Johnson, C. V., Carter, K. C., Singer, R. H., and Lawrence, J. B. (1991). Genetic Analysis Techniques and Applications, 8, 41.

Lawrence, J. B. 1990. A fluorescence in situ hybridization approach for gene mapping and the study of nuclear organization, p. 1. In Editor (ed.), Genome Analysis Volume 1: Genetic and Physical Mapping, Cold Spring Harbor Press, Cold Spring Harbor NY.