Alternative labeling strategies for proteins and RNA (Spinach, .....)
Optimization of tag sizes (length of MS2 cassette....)
Imaging limits in model systems (yeast, non-adherent cells, worms and flys) including optimization of mounting media
Basic molecular biology (Alexa report)
Image processing [3D representation, state analysis , data mining,…)
Calibration / development of microscope parts and performance (stability, registration, synchronization, temp control, multi-channel imaging)
Compare imaging modalities for image processing and registration (test beads vs. NPC vs DNA / gain to photon calibration / sampling and resolution ratios [Nyquist and sub Nyquist imaging conditions]
