Postdoctoral
position in cryo-EM and translation regulation ‒ from bacteria to neurons
Korostelev
Lab, University of Massachusetts Medical School
We study how the ribosome interacts with
numerous cellular components to achieve high accuracy and efficiency of gene
expression. Wed like to understand the key roles of translation and ribosomes
in: (1) Neurodevelopment and neurodegenerative diseases; (2) Bacterial stress
signaling and gene regulation (e.g. coupling with transcription); and
other aspects of gene expression (https://www.umassmed.edu/korostelev-lab/). We use a range of approaches,
including cryo-EM and biochemistry (see selected references below).
Our laboratory is housed at the RNA
Therapeutics Institute, a vibrant and highly interactive research community: https://www.umassmed.edu/rti/about-the-rti/faculty-members/.
Our colleagues are interested in nearly all aspects of RNA biology, from
small to large RNAs to RNA chemistry and therapeutics development.
We
seek a highly motivated person who very recently received a PhD,
or will soon receive a PhD.
Applications sent prior to the dissertation defense date are welcome.
If
you are interested in using single-particle cryo-EM and in situ (cellular) EM to elucidate cellular mechanisms, submit the
following information to ann.powers@umassmed.edu:
1) Your CV (including your
publication list);
2) Cover letter (less than 2
pages) describing:
(i) your previous work - focusing on your most
interesting finding(s),
(ii) your scientific interests,
(iii) which aspects of translation
regulation are of interest to you.
3)
Email addresses of three
references.
Selected
publications (accessible via the provided links):
Carbone CE, Demo G, Madireddy R, Svidritskiy E,
Korostelev AA. ArfB can displace mRNA to rescue
stalled ribosomes. Nature Commun.
2020.
Loveland AB, Demo G, Korostelev AA.
Cryo-EM of elongating
ribosome with EF-Tu GTP elucidates tRNA proofreading.
Nature. 2020.
Loveland AB, Svidritskiy
E, Susorov D, Lee S, Park A, Demo G, Gao FB, Korostelev AA. Ribosome inhibition by
C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM.
bioRxiv. 2020.
Susorov D, Egri
S, Korostelev AA. "Termi-Luc: a versatile assay to
monitor full-protein release
from ribosomes. RNA. 2020.
Svidritskiy
E, Demo G, Loveland AB, Xu C, Korostelev AA. Extensive ribosome and RF2
rearrangements during translation termination.
eLife. 2019.
Loveland AB, Demo G, Grigorieff N,
Korostelev AA. Ensemble cryo-EM elucidates
the mechanism of translation fidelity. Nature. 2017.
Demo G, Rasouly
A, Vasilyev N, Svetlov V,
Loveland AB, Diaz-Avalos R, Grigorieff N, Nudler E, Korostelev AA. Structure of RNA polymerase bound to ribosomal 30S subunit.
eLife. 2017.
Abeyrathne
PD, Koh CS, Grant T, Grigorieff N, Korostelev AA. Ensemble cryo-EM uncovers
inchworm-like translocation of a viral IRES through the ribosome.
eLife. 2016.
Loveland AB, Bah E, Madireddy R,
Zhang Y, Brilot AF, Grigorieff N, Korostelev AA. RibosomeRelA
structures reveal the mechanism of stringent response activation. eLife. 2016.