MERFISH sample submission form

Experimenter Name *

Experimenter Email *

PI or Lab Name *

Institution Name *

Speedtype (if UMass Chan Medical School)

Select which instrument your project will be using. * Alpha MERSCOPE

Enter your library ID here (i.e. VZG147). *

Tissue Type (mouse brain, human skin, etc.) *

Do you want to cell boundary stain your tissue? (Please note: cell boundary staining kits are not compatible with smFISH verification runs, only full MERFISH runs.) * Yes No

Select how your samples will be submitted. * Fresh-frozen tissue blocks to be sectioned by the SCOPE Tissue sections already fixed onto MERFISH coverslips

Number of Samples (for more than three, please submit another form) * 1 2 3

Sample Labels (i.e. Sample 1, Sample 2, Sample 3) *

Number of Sections per Coverslip (if submitting coverslips)

Are these samples meant to be used for smFISH verification runs or full MERFISH runs? * smFISH Verification Run(s) Full MERFISH Run(s)

Were the RIN (RNA Integrity Number) values of these samples checked? * Yes No

If these samples were tested for RIN, enter the numbers for each sample here (ex: Sample 1 - 9.2, Sample 2 - 3.1).

Antibody Names if Antibody Staining (ex: antibody 1, antibody 2, antibody 3)

Antibody lot numbers if antibody staining (ex: antibody 1: number 1, antibody 2: number 2, antibody 3: number 3)

Antibody catalogue numbers if you are antibody staining (ex: antibody 1: number 1, antibody 2: number 2, antibody 3: number 3)

Antibody Storage Temperature (if Antibody Staining) Room Temperature 4°C -20°C

Please describe your project goals in a few sentences. *

If you will be comparing these images to images previously acquired in the SCOPE, please enter the date and file name of the previous scans. This will allow us to use the same image settings.

How would you like your data transferred? (i.e. DropBox, hard drive you would supply, etc.) *

Enter any other notes about your project here.

FOR EXTERNAL USERS ONLY: By clicking this button, I confirm that I have an approved Imaging Request Form (formerly called the Biosafety Form) on file with the SCOPE for this sample type. The Imaging Request Form can be downloaded from the Forms and Resources page on the SCOPE website (https://www.umassmed.edu/scope/forms-and-resources/) Yes

The SCOPE images samples as received. We will work with users to obtain the best images possible given the submitted sample quality. Dirt, fibers, air bubbles, non-flat tissue, and other sample quality issues will result in poor images. By using the SCOPE imaging service, users agree to pay all imaging fees, regardless of resulting image quality. * I understand that my image quality depends upon the quality of my samples. I agree to pay the SCOPE imaging fees for this service.

Vizgen MERFISH sample submission

MERFISH Sample Submission Form