Drosophila Media
We prepare 3 different types of media including Low Yeast Brown, Yellow, and High Yeast Brown. The following are the different formulas we use in preparing the media:
| Low Yeast Brown |
Agar |
6.5 g/L |
|
Brewers Yeast |
23.5 g/L | |
|
Cornmeal |
60 g/L | |
|
Molasses |
60 ml/L | |
|
Acid Mix |
4 ml/L | |
|
Tegosept |
0.13% |
| Yellow |
Agar |
7.2 g/L |
|
Nutritional Yeast |
25 g/L | |
|
Cornmeal |
47 g/L | |
|
Dextrose |
100 ml/L | |
|
Tegosept |
0.22% |
| High Yeast Brown |
Agar |
6.5 g/L |
|
Brewers Yeast |
63 g/L | |
|
Cornmeal |
60 g/L | |
|
Molasses |
60 ml/L | |
|
Acid Mix |
4 ml/L | |
|
Tegosept |
0.13% |
NOTES:
- Dextrose is used as the sugar source in the Yellow formulation rather than molasses. Labs conducting behavioral studies frequently use the Yellow formulation. Also, the Yellow formulation tends to be drier than the Brown.
- The High Yeast formulation has approximately 2.5X the amount of yeast as compared with the Low Yeast formulation.