Services

Data Collection

• Cryo-EM imaging at low-dose condition and liquid nitrogen temperature
• High image quality, high throughput
• Automated cryo-ET data collection
• Automated single particle data collection
• Options for staff assist operation and remote control

Sample Preparation

• Prepare cryo-EM grids

Cryo Grid Screening

• Screen cryo grid conditions on auto-loader scope
• Screening will provide low mag atlas, medium mag montages for a few good meshes, and a few images from good ice areas

Nano-particle Morphology Studies

·        Full morphology analysis for samples such as Lipid-based Nano-Particles (LNP) and AAV like particles.

·        Three different magnifications, ~50+ images in total.

·        Option for statistics analysis for size distribution and particle shapes.

Image Processing

• Preliminary data processing for aligning movie frames in fly during data collection
• 3D reconstruction for tomography data
• 3D reconstruction of single particle data

Structure Determination

·        We now have bandwidth to take full projects. You send sample, we provide final structure.

Training

• Basic theory of electron microscopy
• Hands-on training of basic TEM operation using existing EM Core Facility
• Advanced training on TEM operation using SerialEM
• Hands-on training for operating Titan Krios and Talos-Arctica
• Negative stain sample preparation
• Cryo-EM sample preparation

For information on Training, please see Core Director.