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Data availability for Ganesan et al., bioRxiv (2021): All custom scripts have been made available at https://github.com/Jacobson-Lab/Upf-polysomes. Sequencing data that support this study have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) with the accession code GSE1867951058 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186795). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD0295771061 and 10.6019/PXD029577.

Data availability for Ganesan et al., RNA (2022): All custom scripts have been made available at https://github.com/Jacobson-Lab/Upf1-ribosomes. Sequencing data that support this study have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) with the accession code GSE186795 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186795). The mass spectrometry proteomics data have been deposited to the ProteomeX-change Consortium via the PRIDE partner repository (Perez-Riverol et al. 2019) with the data set identifiers PXD029577 and 10.6019/PXD029577.

Data availability for He et al., eLife (2022): Source data associated with figures of northern blotting analyses have been deposited in the Dryad repository (https://datadryad.org/stash) and within that site can be found at https://doi.org/10.5061/dryad.pc866t1px.

Link to data from He et al., Mol. Cell 12:1439-1452, 2003 https://escholarship.umassmed.edu/gsbs_sp/486/ 
This link provides access to the paper's supplementary data that was originally posted on an external server cited in the paper. The original server is no longer active.

Jacobson lab transcriptome for Saccharomyces cerevisiae, from Mangkalaphiban et al., bioRXiv 2020: https://github.com/Jacobson-Lab/yeast_transcriptome_v5

Strains and plasmids described in our papers are available via email request to: robin.ganesan@umassmed.edu