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BD Symphony A5

BD Symphony configuration

The BD FACSymphony™ A5 flow cytometer is a 5-laser benchtop instrument with the ability to acquire up to 30 parameters. It uses fixed-alignment lasers that transmit light through a flow cell to cascade detector arrays. The Symphony has room to grow, as it is upgradeable with up to 7 lasers and up to 50 parameters.

Using the familiar Diva software, the Symphony has a very close similarity to the control features of the BD Celesta. This analyzer is available for self-use for investigators who have completed the training session described on our training page.

BioRad ZE5 Analyzer

BioRad ZE5 Fluorochrome Selection Guide

The ZE5 is a full-featured analyzer with 5 lasers and 30 detectors including dual FCS for simple to complex flow panels. Features include multiple sample formats (5-ml, eppendorfs, and standard or deep-well microplates), chilled sample holder, operational flow rates of 0.025–3.5 μl/sec, up to 100,000 events per second with all parameters enabled, <0.2 μm FSC resolution with small-particle detection module.

The BioRad ZE5 is located in LRB 357 for self-use analysis. Training is avalilable through the Flow Core.

Sony Core MA900 Cell Sorter (BSL-2+/BSC) AND BSL-3 405/488/531/633 (Paired Co-Linear lasers)

Sony MA900 BSL-2+ and BSL-3 with BSC Lasers/Filters

The Sony MA900 cell sorter is contained in it's own Baker BSC. One is housed in our BSL-2+ room in the flow cytometry core's main lab. A second identical MA900 is housed in the BT2 BSL-3 lab. The MA900 is configured with 4 lasers with 12 parameters, currently utilizing 70µ and 100µ disposable sorting chips and capable of 4-way and plate sorting. Please keep in mind that the lasers on the Sony are paired co-linear and fluorescence overlap cannot be separated within each pair (please see the configuration page). This sorter is available for self-sorting for investigators who have completed the training session described on our training page.